abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...

journal_title：Canadian journal of microbiology

authors： White AP,Allen-Vercoe E,Jones BW,DeVinney R,Kay WW,Surette MG

更新日期：2007-01-01 00:00:00

abstract：:To determine the ability of environmental bacteria to metabolize the frequently prescribed fluoroquinolone drug ciprofloxacin, eight Mycobacterium spp. cultures were grown for 4 days in a medium containing sorbitol and yeast extract with 100 mg x L(-1) ciprofloxacin. After the cultures had been centrifuged and the sup...

journal_title：Canadian journal of microbiology

authors： Adjei MD,Heinze TM,Deck J,Freeman JP,Williams AJ,Sutherland JB

更新日期：2007-01-01 00:00:00

abstract：:The effects of pressurized CO2 on the survival of Escherichia coli and the mechanism of cell inactivation were studied. Bacterial cultures were inoculated in nutrient broth and incubated at 30 degrees C for 18 h. Exposure of the cells to CO2 under pressures ranging from 2.5 to 25 MPa and at temperatures between 8 and ...

journal_title：Canadian journal of microbiology

authors： Oulé MK,Tano K,Bernier AM,Arul J

更新日期：2006-12-01 00:00:00

abstract：:We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...

journal_title：Canadian journal of microbiology

authors： Xiang H,Wei GF,Jia S,Huang J,Miao XX,Zhou Z,Zhao LP,Huang YP

更新日期：2006-11-01 00:00:00

abstract：:The effect of fructans on the cultivable faecal community of Bio Breeding rats fed diets containing 5% (m/v) food-grade fructooligosaccharide (FOS) was investigated. Culturing of faecal material using chicory inulin as the sole carbohydrate source revealed the presence of a greater diversity of inulin-utilizing bacter...

journal_title：Canadian journal of microbiology

authors： Gourgue-Jeannot C,Kalmokoff ML,Kheradpir E,Kwan J,Lampi BJ,McAllister M,Brooks SP

更新日期：2006-10-01 00:00:00

abstract：:Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we scre...

journal_title：Canadian journal of microbiology

authors： Jafra S,Przysowa J,Czajkowski R,Michta A,Garbeva P,van der Wolf JM

更新日期：2006-10-01 00:00:00

abstract：:Dietary exposure to heavy metals may have detrimental effects on human and animal health, even at low concentrations. Specific probiotic bacteria may have properties that enable them to bind toxins from food and water. We assessed the interaction of probiotic bacteria with cadmium and lead in vitro as an initial scree...

journal_title：Canadian journal of microbiology

authors： Ibrahim F,Halttunen T,Tahvonen R,Salminen S

更新日期：2006-09-01 00:00:00

abstract：:The open reading frame pp0053, which has a high homology with the sequence of mitochondrial sulfide dehydrogenase (HMT2) conferring cadmium tolerance in fission yeast, was amplified from Pseudomonas putida KT2440 and expressed in Escherichia coli JM109(DE3). The isolated and purified PP0053-His showed absorption spect...

journal_title：Canadian journal of microbiology

authors： Shibata H,Kobayashi S

更新日期：2006-08-01 00:00:00

abstract：:Phialocephala fortinii is a dark septate fungal endophyte that colonizes roots of many host species. Its effect on plant growth varies from being pathogenic to beneficial. The basic biology of this species has received little research, and thus the main objectives of this study were to determine cytological features o...

journal_title：Canadian journal of microbiology

authors： Saito K,Kuga-Uetake Y,Saito M,Peterson RL

更新日期：2006-07-01 00:00:00

abstract：:Fermentative hydrogen production was carried out by Enterobacter cloacae DM11, using glucose as the substrate. The effects of initial substrate concentration, initial medium pH, and temperature were investigated. Results showed that at an initial glucose concentration of 1.0% (m/v), the molar yield of hydrogen was 3.3...

journal_title：Canadian journal of microbiology

authors： Nath K,Kumar A,Das D

更新日期：2006-06-01 00:00:00

abstract：:Ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. All test isolates produced siderophores as indicated by a positive reaction in the FeCl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. Further, the test isolates were compar...

journal_title：Canadian journal of microbiology

authors： Vala AK,Dave BP,Dube HC

更新日期：2006-06-01 00:00:00

abstract：:The ability to rapidly identify and quantify a microbial strain in a complex environmental sample has widespread applications in ecology, epidemiology, and industry. In this study, we describe a rapid method to obtain functionally specific genetic markers that can be used in conjunction with standard or real-time poly...

journal_title：Canadian journal of microbiology

authors： Hynes SS,Chaudhry O,Providenti MA,Smith ML

更新日期：2006-05-01 00:00:00

abstract：:The comparative ability of different methods to assess virulence of Listeria species was investigated in ten Listeria strains. All strains were initially subjected to pulsed-field gel electrophoresis analysis to determine their relatedness. Virulence characteristics were subsequently tested for by (i) determining the ...

journal_title：Canadian journal of microbiology

authors： Chiu S,Vanderlinde PB,Dykes GA

更新日期：2006-04-01 00:00:00

abstract：:For the recently described serotype 15 of biotype I and serotypes 13 and 14 of biotype II of Actinobacillus pleuropneumoniae, fhuA and hgbA were detected by polymerase chain reaction and DNA sequencing. To determine the substrate specificity of the iron receptors FhuA and HgbA and to study their role in the virulence ...

journal_title：Canadian journal of microbiology

authors： Shakarji L,Mikael LG,Srikumar R,Kobisch M,Coulton JW,Jacques M

更新日期：2006-04-01 00:00:00

abstract：:Eight Enterococcus faecium strains isolated from ewe milk and artisanal cheese from northwest Argentina were screened for biotechnological properties relevant to flavour development. The API ZYM test showed absence of proteases, presence of high amounts of peptidases, and high esterase-lipase activities. Low extracell...

journal_title：Canadian journal of microbiology

authors： Abeijón MC,Medina RB,Katz MB,González SN

更新日期：2006-03-01 00:00:00

abstract：:The presence and taxonomy of endophytic bacteria of the entire aerial parts of crocus (Crocus albiflorus), a wildflower native in the Alps, were investigated. A combination of plating of plant macerates, isolation and sequence identification of isolates, and direct 16S rDNA PCR amplification followed by whole-communit...

journal_title：Canadian journal of microbiology

authors： Reiter B,Sessitsch A

更新日期：2006-02-01 00:00:00

abstract：:Two potassium (K)-bearing minerals, Nanjing feldspar and Suzhou illite, were used to investigate K mobilization by the wild-type strain NBT of Bacillus edaphicus, also labeled MPs+, selected for high activity in mobilizing potassium from minerals, and by four of its UV + LiCl mutants, MPs++, MPs+1, MPs+2, and MPs-. In...

journal_title：Canadian journal of microbiology

authors： Sheng XF,He LY

更新日期：2006-01-01 00:00:00

abstract：:To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a me...

journal_title：Canadian journal of microbiology

authors： Latha JN,Rashmi K,Mohan PM

更新日期：2005-12-01 00:00:00

abstract：:Bacterial diversity was assessed in water samples collected from several uranium mining wastes in Ger many and in the United States by using 16S rDNA and ribosomal intergenic spacer amplification retrievals. The results obtained using the 16S rDNA retrieval showed that the samples collected from the uranium mill taili...

journal_title：Canadian journal of microbiology

authors： Radeva G,Selenska-Pobell S

更新日期：2005-11-01 00:00:00

abstract：:Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucl...

journal_title：Canadian journal of microbiology

authors： Cobos-Marín L,Montes-Vargas J,Zumarraga M,Cataldi A,Romano MI,Estrada-Garcia I,Gonzalez-y-Merchand JA

更新日期：2005-11-01 00:00:00

abstract：:Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH(4))(2)SO(4)) or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized with porcine ge...

journal_title：Canadian journal of microbiology

authors： Rivero-García PC,Cruz CV,Alonso PS,Vaca S,Negrete-Abascal E

更新日期：2005-10-01 00:00:00

abstract：:The ultraviolet germicidal irradiation (UVGI) dose necessary to inactivate fungal spores on an agar surface and the efficacy of UVGI were determined for cultures of Stachybotrys chartarum (ATCC 208877). This study employed a UVGI testing unit consisting of four chambers with a 9-W, Phillips, low pressure, mercury UVGI...

journal_title：Canadian journal of microbiology

authors： Green CF,Davidson CS,Scarpino PV,Gibbs SG

更新日期：2005-09-01 00:00:00

abstract：:We partially purified and characterized the cytochrome aa3 from the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense. This cytochrome aa3 showed oxygen consumption activity with N, N, N', N'-tetramethyl-1,4-phenylenediamine and ascorbate as substrates, and also displayed bovine cytochrome c o...

journal_title：Canadian journal of microbiology

authors： Nunoura T,Sako Y,Wakagi T,Uchida A

更新日期：2005-08-01 00:00:00

abstract：:Farnesol, a quorum sensing (QS) signal, is produced by Candida albicans during high density growth and has been found to inhibit morphogenesis. This QS auto-inducing signal was discovered to increase amino acid incorporation by C. albicans when concentrations of farnesol increased to 10 microg/mL in yeast nitrogen bro...

journal_title：Canadian journal of microbiology

authors： Braun PC

更新日期：2005-08-01 00:00:00

abstract：:Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...

journal_title：Canadian journal of microbiology

authors： Law DK,Stoltz J,Henderson AM,Tsang RS

更新日期：2005-07-01 00:00:00

abstract：:Through gas chromatography - mass spectrometry, the presence of oxylipins, mainly 3-hydroxy 9:1 and 3-hydroxy 10:1, was detected in Saccharomycopsis fermentans, Saccharomycopsis javanensis, and Saccharomycopsis vini. The distribution of these compounds was mapped using immunofluorescence microscopy, and they were foun...

journal_title：Canadian journal of microbiology

authors： Sebolai OM,Kock JL,Pohl CH,Botes PJ,Strauss CJ,Van Wyk PW,Nigam S

更新日期：2005-07-01 00:00:00

abstract：:Trichloroethylene (TCE) is a prevalent contaminant of groundwater that can be cometabolically degraded by indigenous microbes. Groundwater contaminated with TCE from a US Department of Energy site in Ohio was used to characterize the site-specific impact of phenol on the indigenous bacterial community for use as a pos...

journal_title：Canadian journal of microbiology

authors： Humphries JA,Ashe AM,Smiley JA,Johnston CG

更新日期：2005-06-01 00:00:00

abstract：:Dermatophytes are a group of fungi that are capable of invading keratinized tissues of humans and other animals. Antifungal susceptibility analysis and genetic studies by random amplification of polymorphic DNA (RAPD), have been used to detect polymorphism as well as determining the possible resistance of dermatophyte...

journal_title：Canadian journal of microbiology

authors： Brilhante RS,Cordeiro RA,Medrano DJ,Monteiro AJ,Sidrim JJ,Rocha MF

更新日期：2005-06-01 00:00:00

abstract：:The role the environment has on populations of Pythium and Mesocriconema spp. was investigated at 5 golf course locations in east central Alabama. Every 4 to 5 weeks soil samples were collected from 3 golf greens on each of the 5 golf courses. Environmental data, including air and soil temperature, pH and relative hum...

journal_title：Canadian journal of microbiology

authors： Allen TW,Han DY,Bowen KL

更新日期：2005-04-01 00:00:00

abstract：:The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...

journal_title：Canadian journal of microbiology

authors： Moore M,Trevors J,Lee H,Leung KT

更新日期：2005-03-01 00:00:00

abstract：:Bacteria associated with 6 habitats of groundnut were evaluated for their broad-spectrum antifungal activity and suppression of collar rot (Aspergillus niger) of groundnut. Three hundred and ninety-three strains were tested against 8 fungal pathogens of groundnut including 5 necrotrophic fungi, Aspergillus flavus, A. ...

journal_title：Canadian journal of microbiology

authors： Kishore GK,Pande S,Podile AR

更新日期：2005-02-01 00:00:00

abstract：:Habitat fragmentation affects the biodiversity and function of aboveground organisms in natural ecosystems but has not been studied for effects on belowground species. In this paper, we consider the diversity of the rhizobia associated with the indigenous legume Dalea purpurea in 3 residual prairie areas in Minnesota ...

journal_title：Canadian journal of microbiology

authors： Tlusty B,van Berkum P,Graham PH

更新日期：2005-01-01 00:00:00

abstract：:Listeria grayi is a nonpathogenic Gram-positive bacterium that demonstrates considerable similarities to other members in the genus Listeria, including the foodborne human pathogen Listeria monocytogenes and the animal pathogen Listeria ivanovii. A rapid diagnostic test to identify and diagnose listeriosis would be va...

journal_title：Canadian journal of microbiology

authors： Liu D,Lawrence ML,Ainsworth AJ,Austin FW

更新日期：2005-01-01 00:00:00

abstract：:Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...

journal_title：Canadian journal of microbiology

authors： Golubev WI,Sampaio JP,Alves L,Golubev NW

更新日期：2004-12-01 00:00:00

abstract：:Filamentous growth of Candida albicans occurs in response to a variety of environmental signals. The C. albicans gene orf19.1944 and its allele orf19.9499 are identical and are predicted to encode an 823-residue, 7-transmembrane-domain protein that has all the expected features of a G-protein-coupled receptor. The pro...

journal_title：Canadian journal of microbiology

authors： Sciascia QL,Sullivan PA,Farley PC

更新日期：2004-12-01 00:00:00

abstract：:A bacterium C1010, isolated from the rhizospheres of cucumbers in fields in Korea, degraded the microbial quorum-sensing molecules, hexanoyl homoserine lactone (HHSL), and octadecanoyl homoserine lactone (OHSL). Morphological characteristics and 16S rRNA sequence analysis identified C1010 as Acinetobacter sp. strain C...

journal_title：Canadian journal of microbiology

authors： Kang BR,Lee JH,Ko SJ,Lee YH,Cha JS,Cho BH,Kim YC

更新日期：2004-11-01 00:00:00

abstract：:The effect of mechanical wounding or foliar diseases caused by Sclerotinia homoeocarpa or Rhizoctonia solani on the epiphytic yeast communities on creeping bentgrass and tall fescue were determined by leaf washing and dilution plating. Total yeast communities on healthy bentgrass and tall fescue leaves ranged from 7.9...

journal_title：Canadian journal of microbiology

authors： Allen TW,Quayyum HA,Burpee LL,Buck JW

更新日期：2004-10-01 00:00:00

abstract：:This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining ...

journal_title：Canadian journal of microbiology

authors： Ferguson C,Kaucner C,Krogh M,Deere D,Warnecke M

更新日期：2004-09-01 00:00:00

abstract：:Our freshwater caulobacter collection contains about 40 strains that are morphologically similar to Caulobacter crescentus. All elaborate a crystalline protein surface (S) layer made up of protein monomers 100-193 kDa in size. We conducted a comparative study of S-layer secretion in 6 strains representing 3 size group...

journal_title：Canadian journal of microbiology

authors： Iuga M,Awram P,Nomellini JF,Smit J

更新日期：2004-09-01 00:00:00

abstract：:Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...

journal_title：Canadian journal of microbiology

authors： Prado SM,Cerdán ME,González Siso MI

更新日期：2004-08-01 00:00:00